Validation of the membrane filtration method for the detection of Vibrio cholerae in marine waters
DOI:
https://doi.org/10.26640/22159045.2022.590Keywords:
Validation, Vibrio, qualitative method, qualityAbstract
This work describes the validation of the membrane filtration method for the detection of Vibrio cholerae in marine waters. The reference method of the Standard Methods for the Examination of Water and Wastewater (23rd edition) section 9260H was taken into account. The selective culture medium was Thiosulfate Citrate Bile Sucrose (TCBS) agar, the differential medium was CHROMagar Vibrio, and 1% Alkaline Peptone Water (APW) was used as the enrichment medium. To confirm the results, string, oxidase and API20E tests were performed. The parameters determined were detection limit, sensitivity, selectivity/specificity, negative and positive rate, precision, accuracy and uncertainty, which are recommended for qualitative methods. The parameters evaluated had an acceptance criterion of 95 %. For the performance parameters, the results obtained met the optimal acceptance criterion. The method is sensitive and specified for the mycobacterium of interest.
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