Isolation of oligotrophics microorganisms oils degraders in the Bay of Cartagena, Colombia
DOI:
https://doi.org/10.26640/22159045.239Keywords:
biodegradation, hydrocarbons, seawater, emulsion y bacteriaAbstract
The Bay of Cartagena is one of the main ports in the Colombian Caribbean Sea and it is characterized by a high maritime transit, which represents a high degree of exhibition contamination by seawater of well coming from the boats that joumey in this port. These waters are characterized by a high content in oil remainders that get to affect the estuarino ecosystem. Water samples were collected in 9 stations to three depths in the water column (surface, means and bottom), The obtained resulta registered that the stations where a greater growth, emulsificación and turbidity was are near vulnerable zone.s of contaminaban by hydrocarbons, like: ECOPETROL-s, ECOPETROL-Bottom, and Buoy 19-5, B27-5, B27-M, 630-S, B30-M and Harbor Society. The identified microorganisms were classified through the composition of their cellular wall by means of dye of Gram, in which it settled down that microbiota dominant in the isolated samples was negativa Gram, with bacillary bacteria' form, the bacillary Coco and compatible with the Pseudomanas sp, sorts sp. and Bacillus subtilis. However, it was possible to be observad compatible badlli and positiva Gram cocco generally with the Staphylococcus sp sort in smaller proportion, originating of the deepest parts from sections of the water column. Through this study we got isolating nativa microorganisms capable of degrading Bay cruda oil as an energy source, in order to forrn a strain conectan microorganisms which may be employed as an alternativa for the biotechnological DIMAR Ilke alternativa of microorganisms on oil spills, which will require future cantonad trials to laboratory scale for subsequent scaling to field as potential contingency response against oil spills in the Bay of Cartagena.Downloads
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References
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[29] Quintero, P. y J.K. Camacho. (2009). Biorremediación de Aguas de la Bahía de Tumaco Contaminadas con Hidrocarburos Utilizando Consorcios Nativos. Centro de Control de la Contaminación CCCP. Proyecto interno.
[30] Hara, A. y K. Syutsubo. (2003). Alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation. In: Environmental Microbiology, 5, 746-753.
[31] Gerdes, B., Brinkmeyer, R., Dieckmann, G. y E. Helmke. (2005). Influence of crude oil on changes of bacterial communities in Arctic sea-ice. In: Arctic, 53, 129-139.
[32] Cui, Z., Lai, Q., Dong, C., y A. Shao. (2008). Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge. In: Environmental Microbiology, 10, 2138-2149.
[33] Gertler, C., Gerdts, G., Timmis, K.N. y P.N. Golyshin. (2009). Microbial consortia in mesocosm bioremediation trial using oil sorbents, slow-release fertilizer and bioaugmentation. In: System, 69, 288-300.
[34] McKew, B., Coulon, F., Osborn, A., Timmis, K. y McGenity. (2007). Determining the identity and roles of oil-metabolizing marine bacteria from the Thames estuary, UK. January 9 (1): 165–176.
[35] Gómez, S, Gutiérrez, D., A.M., Hernández, Zulay, C., Losada, M. y P. Mantilla (2008). Actores bióticos y abióticos que condicionan la Biorremediación por Pseudomonas en suelos contaminados por hidrocarburos. En: Publicación Científica en Ciencias Biomédicas- ISSN: 179
[2] Freire, J. y Labarta, U. (2003). El Prestige: Impactos sobre los recursos y ecosistemas marinos. En: La Huella del Fuel. Ensayos sobre el “Prestige”. Ed. Fundación Santiago Rey Fernández-Latorre (A Coruña), pp. 104-1351-28.
[3] Bergueiro, J. R. y Domínguez, F. (1996). “Evaporación y mezclas de hidrocarburos”. Editorial Bilbilis. Pp. 348.
[4] Lee, K. y Tremblay, G. (1995). “Biorremediation of oiled beach sediments. Assessment of inorganic and organic fertilizers, evolving technologics” Oil spill Conference: pp. 107-112.
[5] Tang, K., Baskaran, V. y Nemati, M. (2009). An overview of microbiology, biokinetics
[6] Narváez-Flórez, S., Gómez, M. y Martínez, M. (2008). Invemar, Bol. Instituto de Investigaciones Marinas y Costeras-Invemar 63 Selección de Bacterias con Capacidad Degradadora de Hidrocarburos Aisladas a Partir de Sedimentos del Caribe Colombiano. Investigaciones Marinas y Costeras. 37 (1) 61-75 Santa Marta,
Colombia.
[7] Atlas M. (1981). Microbial Degradation of Petroleum Hydrocarbons: an Environmental Perspective, Microbiological
Reviews, 45(1), 180-209.
[8] Leahy, J., y Colwell, R. (1990). Microbial Degradation of Hydrocarbons in the Environment. In: Biodegradation, 54(3), 305-315.
[9] Harayama, S., Kishira, H., Kasai, Y y Shutsubo, K. (1999). Petroleum biodegradation in marine environments. In: Journal of Molecular Microbiology and Biotechnology, 1, 63-70.
[10] Jonathan D., Van Hamme., Singh A. y Owen P. Ward. (2003). Recent Advances in Petroleum Microbiology. Microbiology Molecular Biology Reviews. December 67 (4) 503-549.
[11] Canals, M. V. (2005). Biorremediación de suelos contaminados por hidrocarburos: caracterización microbiológica, química y ecotoxicológica. Tesis Doctoral. Universidad de Barcelona. En: Microbiología (Madrid).
[12] APHA-AWWA-WEF- (2012). Standard Methods for the Examination of Water and Wastewater. 22st Edition. p 9-66 9222D.
[13] Parsons-Maita y Lalli C,M. (1984). A Manual of Chemical and Biological Methods for Seawater Analysis, Primera Edición, reprinted 1985,1989 with corrections, Gran Bretaña.
[14] Echeverri, G., Manjarrez G., Paba G y Cabrera M. (2010). Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia NOVA-Publicación Científica en
Ciencias Biomédicas.8 (13): 1-120.
[15] Cañón, M., Tous, G., López, K., López, R. y Orozco, F. (2007). Variación espaciotemporal de los componentes
fisicoquímico, zooplanctónico y microbiológico en la Bahía de Cartagena. En: Boletín Científico CIOH No. 25: 120-130.
[16] Harayama S., Kishira H., Kasai Y Shutsubo K. (1999). Petroleum biodegradation in marine environments. Journal Molecular Microbiology Biotechnology. Ago. 1(1): 63-70.
[17] Batista, S. B., Mounteer, A. H., Amorim, F., y Tótola, M. (2006). Isolation and characterization of biosurfactant/ bioemulsifier-producing bacteria from petroleum contaminated sites. In: Bioresource technology, 97(6), 868-75. doi: 10.1016/j.biortech.2005.04.020.
[18] Araujo, I., Gómez, A., Barrera, M., Angulo N., Morillo G., Cárdenas, C y Herrera, L. (2008). Surfactantes Biológicos en la Biorremediación de agua contaminadas con crudo liviano. En: Interciencia, 33,
(004), 245-250.
[19] Rivera-Cruz, M.C., Ferrera-Cerrato, R., Volke-Haller, V., Fernández-Linares L. y Rodríguez-Vázquez, R. (2002). Adaptation and Selection of Autochthonous Microorganisms in Culture Media Enriched with Crude Petroleum. In: Terra, 20, 423- 434.
[20] Wake, H. (2005). Oil refineries: a review of their ecological impacts on the aquatic environment. In: Estuarine, Coastal and Shelf Science, 62, 131-140.
[21] Garay, J. (2004). National Program of Research, Evaluation, Preservation, Reduction and Control of Sea and Land Base Marine Pollution–PNICM. Plan of action 2004 -2014. Institute of Marine and Coastal Research Jose Benito Vives De Andrei-INVEMAR, Santa Marta, 128 pp.
[22] Franklin, M.P., Mcdonald, I.R., Bourne, D.G., Owens, N.J., Upstill-Goddard, R.C. y J.C. Murrell. (2005). Bacterial diversity in the bacterioneuston (sea surface microla-yer): the bacterioneuston through the loo-king glass. In: Environmental Microbiolo-gy, 7(3), 723-736.
[23] Mendoza, Y., Pérez, G., y G. Lugioyo. (2002). Bacterioplancton de aguas oceánicas al norte de Cuba: distribución espacial. En: Revista Investigaciones Marinas, 23(1), 27-34.
[24] Nair, D., Ferna, F. J., y García, E. (2008). Isolation and Characterization of Naphthalene- Degrading Bacteria from Sediments of Cadiz Area (SW Spain). In: Environmental Toxicology, 576-582.
[25] Tapilatu, Y., Acquaviva, M., Guigue, C., Mi-ralles, G., Bertrand, J., P. Cuny. (2010). Isolation of alkane-degrading bacteria from deep-sea Mediterranean sediments. In: Letters in Applied Microbiology, 50, 234-236.
[26] Bartha R. y R. Atlas. (1971). Degradation and mineralization of petroleum in sea water: Limitation by nitrogen and phosphorous. In: Biotechnology and Bioengineering, 14(3) 309–318.
[27] Head, I.M. y R.P. Swannell. (1999). Biore-mediation of petroleum hydrocarbon con-taminants in marine habitats. In: Current Opinion in Biotechnology, 234-239.
[28] Mckew, B.A., Coulon, F., Yakimov, M.M., Denaro, R., Genovese, M., Smith, C.J., Osborn, A.M., Timmis, K.N. y T.J. McGenity. (2007). Efficacy of intervention strategies for bioremediation of crude oil in marine systems and effects on indigenous hydrocarbonoclastic bacteria. In: Environmental Microbiology, 9, 1562-1571.
[29] Quintero, P. y J.K. Camacho. (2009). Biorremediación de Aguas de la Bahía de Tumaco Contaminadas con Hidrocarburos Utilizando Consorcios Nativos. Centro de Control de la Contaminación CCCP. Proyecto interno.
[30] Hara, A. y K. Syutsubo. (2003). Alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation. In: Environmental Microbiology, 5, 746-753.
[31] Gerdes, B., Brinkmeyer, R., Dieckmann, G. y E. Helmke. (2005). Influence of crude oil on changes of bacterial communities in Arctic sea-ice. In: Arctic, 53, 129-139.
[32] Cui, Z., Lai, Q., Dong, C., y A. Shao. (2008). Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge. In: Environmental Microbiology, 10, 2138-2149.
[33] Gertler, C., Gerdts, G., Timmis, K.N. y P.N. Golyshin. (2009). Microbial consortia in mesocosm bioremediation trial using oil sorbents, slow-release fertilizer and bioaugmentation. In: System, 69, 288-300.
[34] McKew, B., Coulon, F., Osborn, A., Timmis, K. y McGenity. (2007). Determining the identity and roles of oil-metabolizing marine bacteria from the Thames estuary, UK. January 9 (1): 165–176.
[35] Gómez, S, Gutiérrez, D., A.M., Hernández, Zulay, C., Losada, M. y P. Mantilla (2008). Actores bióticos y abióticos que condicionan la Biorremediación por Pseudomonas en suelos contaminados por hidrocarburos. En: Publicación Científica en Ciencias Biomédicas- ISSN: 179
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2012-12-05
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How to Cite
Isolation of oligotrophics microorganisms oils degraders in the Bay of Cartagena, Colombia. (2012). CIOH Scientific Bulletin, 30, 3-12. https://doi.org/10.26640/22159045.239
